Laboratory evaluation of the Ile1, 016 mutation-effect on several life-history parameters of Aedes aegypti

Abstract

Pyrethroid insecticides were intensively applied in Mexico between 2000 and 2009. We evaluated the effect of the Ile1, 016 mutation on several life-history parameters of Ae. aegypti from Merida, Yucatan. Aedes aegypti colonies was established from larvae collected in houses between October and November 2015. Fifty adult females were analyzed for the frequency of the Ile1, 016 mutation at each collection site (n = 3). All biological parameters were evaluated by comparing the control group versus natural population of Ae. aegypti. The frequency of kdr homozygous (Val1, 016) was high in all sites studied, being 76% in Juan Pablo-II, 85% in Chuburna and 87% in Vergel-III. In Ae. aegypti with kdr homozygous were observed delay larval development, higher mortality in late stages, decrease in blood ingested and have a larger body sizes. Our results suggest that Ae. aegypti with kdr homozygous have a diminished reproductive fitness compared to susceptible group.